Cloned (Comment) | Organism |
---|---|
SARS-CoV-2 nsp12 polymerase and nsp7-nsp8 cofactors are expressed using the baculovirus and Escherichia coli expression systems, respectively. The three protein subunits were mixed in vitro to constitute the core polymerase complex | Severe acute respiratory syndrome coronavirus 2 |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
nucleoside triphosphate + RNAn | Severe acute respiratory syndrome coronavirus 2 | - |
diphosphate + RNAn+1 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Severe acute respiratory syndrome coronavirus 2 | P0DTD1 | replicase polyprotein 1ab; SARS-CoV-2 | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
nucleoside triphosphate + RNAn | - |
Severe acute respiratory syndrome coronavirus 2 | diphosphate + RNAn+1 | - |
? | |
nucleoside triphosphate + RNAn | SARS-CoV-2 core polymerase complex has less efficient activity for RNA synthesis and lower thermostability of individual subunits compared with SARSCoV | Severe acute respiratory syndrome coronavirus 2 | diphosphate + RNAn+1 | - |
? |
Subunits | Comment | Organism |
---|---|---|
More | cryo-EM structure of SARS-CoV-2 nsp12-nsp7-nsp8 core polymerase complex. The structure of the SARS-CoV-2 nsp12-nsp7-nsp8 complex is determined at 3.7-A resolution. The SARSCoV-2 polymerase complex consists of the nsp12 catalytic subunit and nsp7-nsp8 cofactors | Severe acute respiratory syndrome coronavirus 2 |
Synonyms | Comment | Organism |
---|---|---|
RDRP | - |
Severe acute respiratory syndrome coronavirus 2 |
SARSCoV-2-core polymerase complex | - |
Severe acute respiratory syndrome coronavirus 2 |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
additional information | - |
lower thermostability of individual nsp7-8-12 subunits of SARS-CoV-2 core polymerase complex compared with SARS-CoV core polymerase complex | Severe acute respiratory syndrome coronavirus 2 |